The National Guidance for Pollution Hotspotting provides the structure for identifying plastic pollution (micro and macro plastic) hotspots and prioritizing interventions. The guide is organized into modules with corresponding tools that guide workflow through a set of overarching questions: 1. Where to act? (Hotspots), 2. What to do? (Interventions), and 3. How to do it? (Instruments). The aim of the guide is to enable stakeholders to systematically address the challenge of plastic pollution. Regions and cities can use this framework to take action in their local context. This guide also helps stakeholders understand which interventions will have the most significant impact on material flows.